High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts

Periploca aphylla (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use medicine, two different extracts from the aerial parts of plant: chloroform P-1, n-butanol P-2 were subjected biological assays screen peroxisome proliferator-activated receptors (PPAR? PPAR?) agnostic, anti-inflammatory, antioxidant, cytotoxic, estrogenic activities. In addition, five bioactive secondary metabolites isolated rutin, chlorogenic acid, caffeic ursolic stigmasterol. P-1 decreased cellular oxidative stress by 47.0% 62.0%, respectively, compared standard drug quercetin, while one compounds rutin PA-1 extract significantly 67.0% quercetin (75.0%). also activated PPAR? agnostic. did not inhibit nuclear factor kappa B inducible nitric oxide synthase activity showed no cytotoxic or estergenic effects on four human cancer cell lines. this study, both standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC sharp compact bands (Rf = 0.09), acid 0.25), 0.39) scanned at ?max 340 nm water: methanol (60:40 v/v) mobile phase. At 539 0.20) stigmasterol 0.48) chloroform: (98:2 as NP-HPTLC Therefore, developed RP- systems are a precise, sensitive, specific analytical tool for quantification PA, which can be phytomarkers taxonomical identification assessment PA.